Important limitations
- Individual claims and source links have not yet completed editorial verification.
- Evidence level, applicable study model, formulation, route and population remain unassigned.
Testagen
Testagen is a bioregulatory peptide primarily affecting the pituitary gland and thyroid.
Dosing & scaling, explained
The moderated mass amounts below are retained for comparison. Near-duplicates, mix-dependent volumes and device markings are excluded. This is educational source context, not a protocol or recommendation.
Mass amounts available for comparison
- Lower range1 mgDose
Recorded example with no verified study backing; shown for amount literacy only.
- Mid range5 mgDose
Recorded example with no verified study backing; shown for amount literacy only.
Only mcg or mg amounts are retained. The calculator derives mL, syringe units or pen clicks from the current vial strength, water volume and device.
No independently verified schedule
Evidence context
No clinical amount or schedule is inferred from this material. Source verification is pending, and every displayed amount remains educational context rather than guidance.
Storage, handling and interpretation
Product identity
Verify the exact compound, formulation and amount against the product label or certificate before calculation.
Storage & handling
Follow the product label, certificate and validated laboratory SOP; no universal storage assumption is applied here.
Prepared solutions
Label concentration and preparation date, use sterile technique where applicable, and follow a validated stability protocol.
Reported protocols for Testagen
What published studies and recent community write-ups report about amounts, timing and course length. 1 clinical, 2 preclinical and 3 community-reported rows, each linked to its source. These are descriptions of what others report, not instructions, and nothing here is medical advice.
| Context | Dose | Frequency | Route | Course | Source |
|---|---|---|---|---|---|
| Clinical1 row | |||||
| Russian dietary supplement registration (oral tablets and capsules)Adult men (registered as an additional source of peptides for men) | 1-2 tablets (0.3 g or 0.16 g) or capsules (0.2 g) | 1-2 times a day with food | Oral | 1 monthNot stated | Community resource2010 |
| The Russian state register lists Testagen as a dietary supplement made by Firma Vita in St Petersburg, registered on 1 March 2010 with a one-month course of 1-2 tablets or capsules once or twice daily. This is a supplement registration, not a drug approval, and the peptide content per tablet is not stated in the record. | |||||
| Preclinical2 rows | |||||
| Preclinical thyroid study in hypophysectomized chickensChickens with the pituitary removed shortly after hatching | Not stated in the English abstract; the peptide was given over a 40-day course | Repeated administration for 40 days | Injection (route not specified in the abstract) | 40 daysNot applicable | Bulletin of Experimental Biology and Medicine (PubMed)2008 |
| After 40 days of Lys-Glu-Asp-Gly or Ala-Glu-Asp-Gly, the birds' thyrotropin and thyroid hormone concentrations rose and thyroid structure recovered. The abstract gives neither the dose nor the route, the model is a surgically induced hormone deficiency in birds, and the paper describes the peptide as pituitary-derived rather than testicular. | |||||
| Preclinical thymus morphology in young and old hypophysectomized birdsChicks and one-year-old birds after pituitary removal | Not stated in the English abstract (same 40-day model as the thyroid work) | Repeated administration over the course | Injection (route not specified in the abstract) | About 40 daysNot applicable | Bulletin of Experimental Biology and Medicine (PubMed)2013 |
| Both tetrapeptides promoted recovery of thymic structure regardless of the birds' age, and in this paper Lys-Glu-Asp-Gly had the stronger effect. This is a histology result in an avian lymphoid organ and says nothing about gonadal or hormonal effects in people. | |||||
| Community-reported3 rows | |||||
| Research-vial 10-20 day course | 1-2 mg | Once daily | Subcutaneous or intramuscular from a 20 mg vial reconstituted in 2 mL (10 mg/mL; 1 mg = 0.1 mL or 10 units) | 10-20 daysCourses rather than continuous use, following the Russian bioregulator convention | Community resource2026 |
| A 2026 dosage chart records that research-chemical vendors and self-reports describe short daily courses of 1-2 mg from 20 mg vials and states there is no validated human dose. It notes that the published literature does not show the peptide raising testosterone. | |||||
| Capsule or injection courses repeated once or twice a year | 1-2 capsules per day (Cytomed product) or 1-2 mg subcutaneously (research vial) | Once daily | Oral capsules, or subcutaneous injection of a reconstituted vial | 10-30 day courses1-2 courses per year | Community resource2026 |
| This 2026 guide separates the swallowed Russian capsule product from research vials and warns against converting capsule counts into vial milligrams. It describes the usual pairing with other Khavinson products (Endoluten, Libidon) and states that there is no Western trial and no evidence of a testosterone effect. | |||||
| Vendor microgram range | 100-300 mcg per day | Once daily | Subcutaneous or sublingual | 10-20 daysRest periods between cycles | Community resource2026 |
| A 2026 review page reports that vendor sites quote 100-300 mcg a day, a figure 5 to 20 times lower than the milligram courses on other sites, and that neither figure comes from a dose-finding study. It states that the animal studies did not specify a route and that no human data exist. | |||||
How a course is commonly described
- Days 1-10 (up to 20): courseCommunity-reported
Community descriptions follow the Russian bioregulator convention of a short daily course rather than continuous use: 1-2 mg a day by injection for 10-20 days, or 1-2 capsules a day of the Russian supplement for a month. No source describes an onset time or any measurable change during the course.
- Rest and repeatCommunity-reported
Guides describe repeating the 10-30 day course once or twice a year, often alongside other Khavinson peptides. The interval is convention copied from the supplement marketing and has no pharmacological basis in any publication.
- Russian supplement label: 1 monthClinical
The registered oral product is taken as 1-2 tablets or capsules once or twice daily with food for one month. The registration is as a dietary supplement for men and does not state a peptide dose or any clinical finding.
- Preclinical comparisonPreclinical
The only experimental courses in the literature ran 40 days in pituitary-less chickens, with hormone and histology outcomes measured at the end, so nothing is known about onset, persistence after stopping, or any human time course. The abstracts do not state the dose, which is why no animal figure can be converted to a human one.
- No human study of synthetic Lys-Glu-Asp-Gly has been published in English, so every course length and dose above is a convention borrowed from the Russian supplement pattern, not a tested regimen.
- The milligram daily doses on some sites and the 100-300 mcg figures on others differ by more than tenfold, and neither comes from dose-finding work. The bird studies that make up the primary literature do not state their dose in the available abstracts.
- The name points to the testes, but the source papers describe a pituitary-derived peptide tested on thyroid and thymus endpoints in birds. Testagen is not an approved drug anywhere; the Russian registration is for a dietary supplement.
Sources for this section (9)
- Effect of tetrapeptides Lys-Glu-Asp-Gly and Ala-Glu-Asp-Gly on the structure and function of the thyroid gland in neonatally hypophysectomized chickens
- Effects of hypophyseal Lys-Glu-Asp-Gly and Ala-Glu-Asp-Gly synthetic peptides on immunity, hemostasis, morphology and functions of the thyroid gland in neonatally hypophysectomized chicken and one-year-old birds
- Effect of peptides Lys-Glu-Asp-Gly and Ala-Glu-Asp-Gly on the morphology of the thymus in hypophysectomized young and old birds
- Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA
Plus 5 community resources (dosing guides, forum threads and vendor write-ups), reported above but not linked.
Overview
Testagen is the name used by Vladimir Khavinson's St Petersburg Institute of Bioregulation and Gerontology for the tetrapeptide Lys-Glu-Asp-Gly (KEDG), sold online as a testicular bioregulator. The handful of indexed papers describe it as synthesized from the amino acid composition of anterior pituitary peptide complexes and test it alongside Ala-Glu-Asp-Gly (Epitalon) in chickens whose pituitary glands were removed, where 40 days of injections raised thyrotropin and thyroid hormone levels and restored thyroid and thymus structure. No study of the synthetic peptide in humans has been published in English, nothing in PubMed links the sequence to testosterone or testicular function, and the compound is not approved as a drug anywhere; in Russia a product called Testagen is registered as a dietary supplement for men. The dosing figures that circulate online are copied from the Russian supplement course pattern rather than from any dose-finding work.
What researchers are exploring
These themes organize the educational information currently available. Individual claims remain under source review and are not treatment claims.
- 1
Research on Testagen
The Thyroid Gland and its Importance The thyroid gland, a vital component of the endocrine system, is central to various bodily functions such as metabolism, growth, and reproductive processes.…
What is known and what still needs verification
Available context is organized without assigning unsupported study models or evidence grades.
- Not graded
Research on Testagen
The Thyroid Gland and its Importance The thyroid gland, a vital component of the endocrine system, is central to various bodily functions such as metabolism, growth, and reproductive processes.…
Amounts recorded for Testagen
These amount records are independent from vial strength, reconstitution volume and measuring device. A recorded source is not the same as study backing, and these values are not recommendations.
- 1Dose · lower endpointSign in for schedule, duration and source context.1 mg
- 2Dose · upper endpointSign in for schedule, duration and source context.5 mg
Chemistry identifiers
Sequence, synonyms and registry identifiers as recorded in PubChem, the National Library of Medicine's open chemistry database. Formula, mass and CAS number are summarized at the top of the page.
Sequence
Lys-Glu-Asp-Gly
Also listed as
H-Lys-Glu-Asp-Gly-OH (PubChem record title), KEDG (one-letter abbreviation used in the literature), Testagen (Khavinson program name), Lys-Glu-Asp-Gly, SCHEMBL31734738
InChIKey
HKEYFJLTNCUNAD-DCAQKATOSA-N
IUPAC name
(4S)-5-[[(2S)-3-carboxy-1-(carboxymethylamino)-1-oxopropan-2-yl]amino]-4-[[(2S)-2,6-diaminohexanoyl]amino]-5-oxopentanoic acid
Limitations and unknowns
Sources
1 link carried over from the earlier page, awaiting verification.