Peptides >TB-500 (Thymosin Beta-4)

TB-500 (Thymosin Beta-4)

Thymosin Beta-4, consisting of a 43 amino acid peptide sequence, has demonstrated its potential in animal models for various beneficial effects. These include the enhancement of blood vessel growth, the regulation of wound healing processes, the reduction of inflammation, and the mitigation of oxidative damage in vital areas such as the heart and central nervous system. Thymosin Beta-4 plays a significant role in safeguarding tissues, facilitating repair, promoting tissue regeneration, and aiding in the restructuring of injured or damaged tissues. It is also a subject of active research interest in the field of anti-aging, given its potential to support longevity and overall health.

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1. TB-500 (Thymosin Beta-4)


2. Molecular Structure of TB-500 (Thymosin Beta-4)


3. TB-500 Research Studies

TB-500 (Thymosin Beta-4)

Thymosin Beta 4, also known as TB-500, is a synthetic version of a naturally occurring 43-amino acid peptide found in a wide range of human and animal cells.

Research, such as a 2010 study published in the Annals of the New York Academy of Sciences, has indicated the potential of TB-500 in facilitating the repair of cardiac muscle after injuries like myocardial infarction (heart attack). TB-500 was found to be promising in cases where stem cell therapy had limitations. It demonstrated the ability to inhibit the death of myocardial cells, stimulate the growth of blood vessels, and activate processes within the heart that promote healing following injury. This suggests that TB-500 could be a groundbreaking agent for actively restoring damaged cardiac muscle after a heart attack. Previous studies in mice conducted in 2004 also supported the idea of TB-500 promoting cardiomyocyte migration, survival, and repair of myocardial damage.

In cystic fibrosis patients, filamentous actin (F-actin) can contribute to thickened sputum, which worsens their condition. TB-500 was studied in a group of cystic fibrosis patients, and the results showed that when combined with dornase alfa, TB-500 led to a dose- and time-dependent decrease in sputum cohesivity. This combination therapy resulted in a remarkable 71% improvement in mucociliary transport of mucus and a 44% improvement in cough transport of mucus.

TB-500 is known to stimulate myoblasts and myocytes, which are cells involved in muscle generation. Following muscle injury, mitochondrial RNA levels of TB-500 have been observed to increase, contributing to the regeneration of muscle fibers and addressing inflammation in the injured area. This data suggests that muscle injury triggers the local production of TB-500, which in turn promotes the migration of incoming myoblasts, expediting the process of skeletal muscle regeneration.

Molecular Structure of Adipotide

Sequence: Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser 
Molecular formula: C212H350N56O78S 
Molar Mass: 4963.4408
CAS number: 77591-33-4 
PubChem: CID 16132341 
Synonyms: Thymosin Beta 4

TB-500 Research Studies

Several studies have explored the potential benefits of Thymosin Beta 4 (TB-500) across various health conditions:

  1. Diabetic Eye Surgery: A 2009 study utilizing TB-500 eye drops at a concentration of 0.01% demonstrated accelerated healing following eye surgery in diabetic patients, who often face complications in healing, particularly with diabetic retinopathy. The study indicated no serious side effects, though some patients reported mild issues like headache, dizziness, and insomnia.

  2. Cystic Fibrosis and Mucociliary Transport: TB-500 was studied in cystic fibrosis patients and, when combined with dornase alfa, resulted in a significant reduction in sputum cohesivity. This combination therapy improved mucociliary transport of mucus and cough transport in these patients.

  3. Liver Disease: TB-500 was investigated in the context of chronic hepatitis B combined with nonalcoholic fatty liver disease (NAFLD). Although it did not directly correlate with HepB Virus levels or liver function tests, it showed a negative correlation with inflammation and fibrosis scores, suggesting potential benefits in certain liver diseases.

  4. Safety and Tolerance: A human safety and tolerance study conducted in 2010 found that intravenous (IV) doses of TB-500 ranging from 42mg to 1260mg daily for 14 days had no treatment-related adverse effects or dose-related toxicity.

  5. Immune Response and Respiratory Virus: A study involving healthy volunteers exposed to rhinovirus (common cold virus) found an increase in serum cortisol, thymosin alpha 1, and TB-500 on the fifth day post-exposure. This correlated with an increase in various immune cells, indicating a cooperative effect between thymosin and the immune response to respiratory viruses.

  6. Kidney Disease: Research in mice with and without kidney disease revealed that low levels of natural TB-500 were associated with accelerated kidney disease progression. This suggests that endogenous TB-500 levels play a protective role in kidney health.

  7. Skin and Wound Healing: TB-500 has shown promise in accelerating the healing of skin in various conditions, including burns, diabetic ulcers, pressure ulcers, and more. It enhances angiogenesis, exhibits anti-inflammatory properties, and increases platelet aggregation at wound sites.

  8. Meta-Analysis: A 2015 meta-analysis of studies on TB-500 highlighted its potential in a wide range of disease processes, including tissue regeneration, heart repair after heart attacks, brain healing post-stroke and trauma, improvements in kidney and liver diseases, spinal cord and bone injuries, and mitigating the effects of aging and viral infections.

  9. Fracture Healing: TB-500 treatment in mice with fractures led to significantly improved strength and stiffness in healed fractures compared to untreated mice. Imaging also revealed better-healed injury sites.

  10. Spinal Cord Injury: In a study involving rats with spinal cord injuries, TB-500 administration led to marked improvements in locomotor and behavioral assessments, reduced inflammatory cytokines, scar size, and increased levels of myelin proteins, suggesting potential benefits for humans with spinal cord injuries.

  11. Traumatic Brain Injury (TBI): TB-500 has been shown to have neuroprotective and neurorestorative effects in rat models of traumatic brain injury. It promotes increased blood flow, the generation of new brain cells, and the formation of new connections between brain cells.

  12. Multiple Sclerosis (MS): In rat models, TB-500 administration increased the number of newly generated oligodendrocytes, reduced axonal damage, and promoted remyelination of axons, correlating with functional improvement in multiple sclerosis.

These studies collectively suggest that TB-500 may hold promise in various medical applications and therapeutic interventions for a wide range of health conditions. However, further research, including clinical trials in humans, is needed to fully understand its potential and safety.


Article Author

The above literature was researched, edited and organized by Dr. Logan, M.D. Dr. Logan holds a doctorate degree from Case Western Reserve University School of Medicine and a B.S. in molecular biology.


The product information featured on this website pertains exclusively to in-vitro studies. In-vitro studies, also known as ‘in glass’ studies, are conducted outside of living organisms. It’s important to emphasize that these products do not constitute medicines or drugs and have not received FDA approval for the prevention, treatment, or cure of any medical conditions, ailments, or diseases. It is crucial to note that the introduction of these products into the bodies of humans or animals is strictly prohibited by law.